Cloning Multiple Choice Questions And Answers

21. The first step in cloning a gene is to
A) insert a plasmid into a bacterium
B) isolate the DNA from the organism that contains the desired gene
C) plate cells on agar
D) treat plasmids with restriction enzymes
Ans: b

22. Plasmids are put into bacterial cells by
A) restriction enzymes
B) DNA ligase
C) binding of cohesive sticky ends
D) transformation
Ans: D

23. Restriction enzymes
A) cut donor DNA evenly so smooth edges result
B) cut donor DNA but do not affect plasmids
C) make staggered cuts at specific sequences in DNA in both donor DNA and plasmid
D) are used to incorporate plasmids into bacterial host cells
Ans: C

24. DNA fragments can hybridize with plasmids that have been cut by the same restriction enzyme.
A) True
B) False
Ans: A

25. DNA ligase enzyme joins the cohesive ends of the desired DNA fragment with the cohesive ends of the plasmid.
A) True
B) False
Ans: A

26. What is the name of the first sheep to be cloned from an adult sheep?
  A: Polly
  B: Dolly
  C: Molly
Ans: B


27. When was the first animal cloned?
  A: 1960
  B: 1970
  C: 1975
Ans: B


28. What was the first animal to be cloned?
  A: A bee
  B: A frog
  C: A weasel
Ans: B


29. When was the first test tube baby born?
  A: 1953
  B: 1978
  C: 1983
Ans: B


30. When were the first human cells cloned?
  A: 1981
  B: 1993
  C: 2002
Ans: B

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